Show simple item record

dc.contributor.authorMould, Arne
dc.contributor.authorDe Los Angeles, Alejandro
dc.contributor.authorTunbridge, Elizabeth
dc.identifier.citationDavid J Wright, Nicola Hall, Naomi Irish, Angela L Man, Will Glynn, Arne Mould, Alejandro De Los Angeles, Emily Angiolini, David Swarbreck, Karim Gharbi, Elizabeth M Tunbridge, Wilfried Haerty. Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes. bioRxiven
dc.description.abstractAlternative splicing (AS) is a key mechanism underlying cellular differentiation and a driver of complexity in mammalian neuronal tissues. However, understanding of which isoforms are differentially used or expressed and how this affects cellular differentiation remains unclear. Long read sequencing allows full-length transcript recovery and quantification, enabling transcript-level analysis of AS processes and how these change with cell state. Here, we utilise Oxford Nanopore Technologies sequencing to produce a custom annotation of a well-studied human neuroblastoma cell line and to characterise isoform expression and usage across differentiation. We identify many previously unannotated features, including a novel transcript of the voltage-gated calcium channel subunit gene, CACNA2D2. We show differential expression and usage of transcripts during differentiation, and identify a putative molecular regulator underlying this state change. Our work highlights the potential of long read sequencing to uncover previously unknown transcript diversity and mechanisms influencing alternative splicingen
dc.description.sponsorshipSupported by the NIHRen
dc.titleLong read sequencing reveals novel isoforms and insights into splicing regulation during cell state changesen

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record