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Roadblock: improved annotations do not necessarily translate into new functional insights
(2021-11)
The advent of cost-effective high-throughput nucleotide sequencing means that information about the transcriptome is accruing at an exponential rate, rapidly refining our understanding of the diversity of gene products. ...
Accurate expression quantification from nanopore direct RNA sequencing with NanoCount
(2021-11)
Accurately quantifying gene and isoform expression changes is essential to understanding cell functions, differentiation and disease. Sequencing full-length native RNAs using long-read direct RNA sequencing (DRS) has the ...